Premium
Repression of ALA synthase by heme and zinc‐mesoporphyrin in a chick embryo liver cell culture model of acute porphyria
Author(s) -
RUSSO S. M.,
PEPE J. A.,
CABLE E. E.,
LAMBRECHT R. W.,
BONKOVSKY H. L.
Publication year - 1994
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1994.tb02184.x
Subject(s) - heme , heme oxygenase , porphyria , biochemistry , chemistry , endocrinology , medicine , enzyme , biology
. We characterize a liver cell culture model for acute hepatic porphyrias that recapitulates the biochemical features of the human syndrome. In chick embryo liver cells in primary culture exposed to glutethimide and 4,6‐dioxoheptanoic acid, heme alone produced a transient dose‐dependent decrease in 6‐aminolevulinate synthase and a concomitant increase in heme oxygenase. The addition of low concentrations of zinc‐mesoporphyrin (50–200 nM), an inhibitor of heme oxygenase, led to more prolonged decreases in activity of the synthase and to an additive effect with heme. These effects of zinc‐mesoporphyrin were associated with prolonged inhibition of heme oxygenase. These results suggest that the treatment of choice of acute porphyric syndromes may be the combination of low doses of heme and zinc‐mesoporphyrin or another similarly non‐toxic inhibitor of heme oxygenase.