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Decreased clearance of uraemic and mildly carbamylated low‐density lipoprotein
Author(s) -
HÖRKKÖ S.,
HUTTUNEN K.,
KERVINEN K.,
KESÄNIEMI Y. ANTERO
Publication year - 1994
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1994.tb00974.x
Subject(s) - chemistry , tbars , low density lipoprotein , lipid peroxidation , clearance , medicine , clearance rate , endocrinology , catabolism , lipoprotein , thiobarbituric acid , in vivo , biochemistry , cholesterol , metabolism , antioxidant , microbiology and biotechnology , biology , urology
. Low‐density lipoprotein (LDL) was in vitro carbamylated with potassium cyanate and the clearance was studied in man. A minor carbamylation of LDL decreased the clearance of LDL by 41% (94% of amino groups free) and by 18% (90% of amino groups free). When LDL was extensively carbamylated its clearance was substantially accelerated. Moreover, the clearance of LDL isolated from 14 haemodialysis patients (uremic‐LDL) was studied in rabbits. Uraemic‐LDL, injected into rabbits simultaneously with the LDL of a healthy control subject, was cleared more slowly than the control‐LDL (difference in fractional catabolic rate –6·5%, P = 0·02). We also examined the lipid peroxidation of the carbamylated LDL by measuring the amount of thiobarbituric‐acid reactive substances (TBARS) and formation of conjugated dienes during exposure of carbamylated LDL to 5 μ M Cu 2+ . The carbamylated and native LDL had similar lipid peroxidation and propensity for oxidation. In summary, both the uraemic‐LDL and minimally carbamylated LDL had a decreased clearance in vivo , which may contribute to the accelerated atherosclerosis in uraemic patients.