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Possible involvement of β‐PKC rather than that of α‐PKC in differentiation of 3T3–L1 cells to adipocytes
Author(s) -
UEDA H.,
ISHIDA Y.,
TANIGUCHI H.
Publication year - 1993
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1993.tb02040.x
Subject(s) - protein kinase c , 3t3 l1 , ibmx , 3t3 cells , alpha (finance) , microbiology and biotechnology , endocrinology , adipocyte , chemistry , cellular differentiation , medicine , biology , cell culture , kinase , adipose tissue , biochemistry , forskolin , transfection , stimulation , gene , genetics , construct validity , nursing , patient satisfaction
. The change of subspecies of protein kinase C (PKC) was studied in 3T3—L1 cells in terms of their differentiation to adipocytes. 3T3—L1 cells feasible to differentiate to adipocytes by exposure to 3–isobutyl‐1–methylxanthine (IBMX) and dexamethasone had both α‐ and β‐PKC. However, 3T3–Ll cells unfurnished with such feasibility had only α‐PKC. α‐PKC, therefore, seems to be more deeply involved in differentiation of 3T3–LI cells to adipocytes than α‐PKC.