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Pancreatic beta‐cell function and interleukin‐1β in plasma during the acute phase response in patients with major burn injuries
Author(s) -
WOGENSEN L.,
JENSEN M.,
SVENSSON P.,
WORSAAE H.,
WELINDER B.,
NERUP J.
Publication year - 1993
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1993.tb00780.x
Subject(s) - proinsulin , beta (programming language) , medicine , endocrinology , beta cell , acute phase protein , insulin , interleukin , tumor necrosis factor alpha , chemistry , biology , cytokine , inflammation , islet , computer science , programming language
. Animal experiments demonstrate that inter‐leukin‐lβ (IL‐1β) is beta‐cell cytotoxic in vitro and inhibits insulin secretion in vivo . However, it is unknown if IL‐Iβ affects beta‐cell function in man. Since IL‐1β and other cytokines are main mediators of the acute phase response, the objectives of the present study were to examine beta‐cell function in patients with major burn injuries, and to test if changes in beta‐cell function correlated to systemic levels of IL‐1β and tumour necrosis factor α (TNFα). We established and validated an IL‐1β assay measuring free and protein bound IL‐1β; protein bound IL‐1β was detached from the IL‐1β specific binding protein by acidification, rendering it accessible for the employed antibody. The IL‐1β specific binding protein (43–60 kDa) was found in serum and plasma from all tested patients and normal subjects. Survivors of burn injuries had a stimulated beta‐cell function, whereas non‐survivors had an impaired beta‐cell function as indicated by an increased plasma concentration of proinsulin, and an increased proinsulin/insulin ratio. In addition, non‐survivors had significantly increased plasma levels of TL‐1β. However, we could not demonstrate any correlation between C‐peptide, proinsulin, insulin or proinsulin/insulin ratio and plasma concentration of IL‐1β. In conclusion, beta‐cell function abnormalities are evident in patients with major burn injuries, and a high plasma level of IL‐1β correlates with a fatal outcome. However, the present study did not provide evidence for the hypothesis that beta‐cell function is influenced by circulating IL‐1β or TNFα during the acute phase response.