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Lipid synthesis and apolipoprotein gene expression in hepatocytes in primary culture from (puromycin‐induced) nephrotic rats
Author(s) -
BOUSTANI S. EL,
RIBEIRO A.,
JANVIER B.,
LORIETTE C.,
BENSMAN R.,
DRUET P.,
CHAMBAZ J.,
MANGENEY M.
Publication year - 1993
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1993.tb00764.x
Subject(s) - medicine , puromycin , endocrinology , apolipoprotein b , nephrotic syndrome , overproduction , secretion , hyperlipidemia , lipogenesis , hepatocyte , very low density lipoprotein , chemistry , chylomicron , nephrosis , messenger rna , biology , cholesterol , in vitro , lipoprotein , protein biosynthesis , biochemistry , lipid metabolism , gene , diabetes mellitus
. Primary culture of hepatocytes from puromycin aminonucleoside‐induced nephrotic rats were used to discriminate between the hepatic and extra‐hepatic contribution to the hyperlipidemia occurring in the nephrotic syndrome. De novo lipogenesis and utilization of exogenous fatty acids were not modified in nephrotic hepatocytes as compared to controls. In contrast 2.2 and 5.3‐fold more triacylglycerol and phospholipids were secreted respectively by nephrotic hepatocytes than by controls. Triacylglycerol overproduction was not associated with an increase either in apo B mRNA level or in ape B synthesis or secretion measured by [ 35 S]‐methionine incorporation and immunoprecipitation. We also observed a significant increase in apo A1 and apo E synthesis and secretion by nephrotic hepatocytes. This increase was correlated with a greater amount of apo A1 and apo E mRNA than in controls. The overproduction of apo A1 and apo E by nephrotic hepatocytes might intervene in the clearance of plasma lipoproteins and the redistribution of plasma cholesterol.