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Alternative splicing of human G6PD messenger RNA in K562 cells but not in cultured erythroblasts
Author(s) -
CAPPELLINI M. D.,
TAVAZZI D.,
MONTEMUROS F. MARTINEZ DI,
SAMPIETRO M.,
GAVIRAGHI A.,
CARANDINI D.,
FIORELLI G.
Publication year - 1993
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1993.tb00760.x
Subject(s) - k562 cells , rna splicing , messenger rna , alternative splicing , rna , microbiology and biotechnology , biology , chemistry , genetics , gene
. The biochemical properties of glucose‐6‐phosphate dehydrogenase (G6PD) vary in different tissues, and different protein isoforms of the enzyme have been described. Alternative splicing of G6PD intron VII has been detected in transformed lympho‐blasts, granulocytes and spermatocytes; the function of this mRNA species is still unknown. We developed a PCR for detecting alternatively spliced G6PD mRNA in K562 and in erythroblasts at different stage of maturation obtained from human peripheral BFU‐E in order to evaluate a possible physiological role during erythroid maturation. Trace events of alternative splicing of G6PD intron VII sequences were observed in K562 cells but not in BFU‐E‐derived erythroid precursors; we consider this phenoemenon a non‐functional activity in the cells analysed.