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Angiotensin converting enzyme activity in the serum, lung and kidney of diabetic rats
Author(s) -
ERMAN A.,
DYK D. J. VAN,
CHENGAL B.,
GILER I. D. S.,
ROSENFELD J. B.,
BONER G.
Publication year - 1993
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1993.tb00722.x
Subject(s) - medicine , endocrinology , diabetes mellitus , kidney , streptozotocin , insulin , lung , angiotensin converting enzyme , renin–angiotensin system , enzyme assay , renal function , plasma renin activity , enzyme , chemistry , blood pressure , biochemistry
. Diabetes Mellitus in its early stages, is associated with kidney enlargement and increased glomerular filtration rate in humans and in rats. The present study was designed to clarify the direct effect of diabetes on serum and tissue angiotensin converting enzyme (ACE) activity in streptozotocin‐induced diabetic rats. Serum ACE activity, as determined using a radiometric assay, was significantly increased in the diabetic rats ( n = 15) 14 days after induction of diabetes (670 ± 31 vs. 506±14 nmol ml ‐1 min ‐1 ). Lung ACE activity, but not renal, was significantly elevated at 7 and 14 days by 29 and 46%, respectively. Plasma renin activity in the diabetic rats was decreased at 7 and 14 days by 41 and 78%, respectively. Incubations of lung slices in the presence of glucose at different concentrations did not affect in‐vitro release of the enzyme. Administration of insulin (8 units kg ‐1 ) to diabetic rats ( n = 6) on the 4th day for 11 days reduced ACE activity to values below control. Thus, serum and lung ACE activity is increased in the diabetic rat and reduced upon insulin treatment.