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No evidence for feedback inhibition of hepatic apolipoprotein B (apo B) production after extracorporeal low density lipoprotein precipitation as determined by [I‐ 13 C]leucine infusion in normal volunteers
Author(s) -
ARENDS J.,
BIER D. M.,
SCHÄFER G.,
ARMSTRONG V. W.,
THIERY J.,
SEIDEL D.,
SCHAUDER P.
Publication year - 1993
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1993.tb00721.x
Subject(s) - apolipoprotein b , very low density lipoprotein , medicine , endocrinology , chemistry , leucine , lipoprotein , apheresis , metabolism , low density lipoprotein , cholesterol , biochemistry , platelet , amino acid
. To determine the impact of an acute reduction of the circulating mass of apolipoprotein B (apo B) on apo B metabolism we studied six healthy male volunteers before (day 0), 1 day after (day 2), and 7 days after (day 8) an LDL apheresis treatment which reduced apo B mass by 59%. Appearance of newly synthesized apo B in plasma VLDL and LDL was studied using a primed‐constant infusion of [I‐ 13 C]‐leucine. VLDL apo B pool size and fractional VLDL apo B production rate calculated using a one‐compartment model were similar on all 3 study days. Absolute VLDL apo B production was not statistically different throughout the study (19.7±12.3, 19.5 ± 7.5, 29.1 ± 17.7 mg kg ‐1 day ‐1 ). LDL apo B fractional production rate was increased on day 2 (0.38 ± 0.17, 0.68±0.08, 0.37±0.06 pools day ‐1 on days 0, 2, and 8; P <0.01). Absolute LDL apo B production, however, remained constant throughout the study (10.8 ± 3.3, 11.0±1.9, 10.8 ± 3.1 mg kg ‐1 day ‐1 ). We conclude that in healthy male volunteers acute reduction of the circulating apo B mass by LDL apheresis does not affect apo B metabolism significantly.