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Raised concentrations of the carboxy terminal propeptide of type IV (basement membrane) procollagen (NC1) in serum and urine of patients with glomerulonephritis
Author(s) -
KELLER F.,
SER Y. LYREAL,
SCHUPPAN D.
Publication year - 1992
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1992.tb01823.x
Subject(s) - medicine , creatinine , renal function , endocrinology , radioimmunoassay , proteinuria , urine , procollagen peptidase , type iv collagen , glomerulonephritis , urinary system , glomerular basement membrane , kidney , chemistry , laminin , biochemistry , cell
. Type IV collagen is a major component of the glomerular and tubular basement membrane. We used a specific radioimmunoassay to determine (mean ± SD) the concentration of carboxy terminal non‐collagenous fragment (NCI) of type IV procollagen in the serum (normal 6.0± 1.2 ng ml ‐1 ) and urine (normal 1.5 ± 2.0 ng ml ‐1 )of 142 patients with various kidney diseases. The 15 patients with active glomerulonephritis displayed (anova, Scheffé test) significantly elevated NCI values in their serum (14 ± 8.2 ng ml ‐1 ) as compared with the 32 patients with chronic interstitial nephritis (7.8 ± 30 ng ml ‐1 ), the 17 patients with various other chronic kidney diseases (8.1±2.4 ng ml ‐1 ) and the 23 ambulatory kidney transplant patients (9.1 ± 1.7 ng ml ‐1 ). The highest serum NCI concentrations were found in nine patients with mem‐branoproliferative glomerulonephritis (16± 9.4 ng ml ‐1 ). Sequential serum NCI concentrations in the one patient with active Goodpasture's syndrome were marginally elevated (< 11 ng ml ‐1 ). Serum NCI did not increase with acute interstitial rejection episodes in six kidney transplant patients. The highest urinary NCI concentrations were found in seven patients with minimal change glomerulonephritis (7.5 ± 3.2 ng ml ‐1 ). No correlation was found between serum NCI and serum creatinine, NCI and creatinine clearance, or renal NCI clearance and creatinine clearance. There was a significant correlation between serum NCI and proteinuria. Serum and urinary NCI concentrations were elevated independently from renal function, thus indicating intrinsic renal disease. We conclude that serum and urinary NCI may be useful parameters for distinguishing active damage to the glomerular and tubular basement membrane from other kidney diseases.