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Leukotriene B4 level in stimulated blood neutrophils and alveolar macrophages from healthy and asthmatic subjects. Effect of beta‐2 agonist therapy
Author(s) -
PACHECO Y.,
HOSNI R.,
CHABANNES B.,
GORMAND F.,
MOLIERE P.,
GROSCLAUDE M.,
PIPERNO D.,
LAGARDE M.,
PERRINFAYOLLE M.
Publication year - 1992
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1992.tb01437.x
Subject(s) - bronchoalveolar lavage , salbutamol , leukotriene , leukotriene b4 , agonist , stimulation , immunology , medicine , endocrinology , incubation , asthma , chemistry , receptor , inflammation , lung , biochemistry
. Leukotriene B 4 levels were measured after stimulation by calcium ionophore A23187: (i) in peripheral neutrophils (PMN) from allergic asthmatics, rhinitis and healthy subjects; (ii) in macrophages collected by bronchoalveolar lavage. LTB 4 levels in PMNs were significantly higher in non‐treated allergic asthmatics and non‐treated subjects with rhinitis compared to controls. Beta‐2 agonist‐treated asthmatics showed a significantly decreased LTB 4 production which was not different from those of controls. In vitro , LTB 4 production decreased significantly after PMN incubation with Salbutamol (10 ‐6 mo11 ‐1 ). LTB 4 produced by AM collected by BAL was measured in non‐treated (n = 5) and treated (n= 11) asthmatics with inhaled beta‐2 agonist. AM collected from all controls and non‐treated asthmatics produced LTB 4 . By contrast, no production of LTB 4 was observed in the treated group. LTB 4 production decreased when normal AM were incubated in vitro with Salbutamol (10 ‐8 mol 1 ‐1 ). These results suggest that biochemical differences occur in PMN and macrophages from subjects treated with beta‐2 agonist, presumably in changing the 5‐lipoxygenase pathway.