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In vitro inhibition of Helicobacter pylori urease: biochemical and ultrastructural analysis
Author(s) -
NILIUS M.,
BODE G.,
LEHNHARDT G.,
MALFERTHEINER P.
Publication year - 1991
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1991.tb01409.x
Subject(s) - helicobacter pylori , urease , ultrastructure , in vitro , microbiology and biotechnology , chemistry , biology , biochemistry , enzyme , genetics , anatomy
. Inhibition of H. pylori urease was studied by means of electron‐microscopy and electrophoretic methods using different urease inhibitors, such as acetohydroxamic‐acid (AHA), L‐ascorbic acid (AsA), copper ions, a combination of L‐ascorbic acid with copper ions and UV light. AHA in two different concentrations and AsA at a concentration of 0·1 mg ml ‐1 showed incomplete inhibition of H. pylori urease activity in our electrophoretic experiments. Only membrane‐bound activity was inhibited with AHA but not the activity localized within the cytoplasma as demonstrated by electron‐microscopy. AsA at a concentration of 0·5 mg ml ‐1 and the combination of copper ions (1 μg ml ‐1 ) with AsA completely inhibited the urease activity as demonstrated by electron‐microscopy and electrophoretic experiments. Cu 2+ ions in high concentrations (100 μg ml ‐1 ) and UV light exposure for more than 4 h induced a complete disintegration of H. pylori. Electrophoresis showed no active protein after UV light exposure of 2 h. Different urease inhibitors tested in this study showed dose‐dependent inhibitory effects on H. pylori urease in vitro.