Premium
The recovery of renal function in rats after release of unilateral ureteral obstruction: the effects of moderate isotonic saline loading
Author(s) -
HARRIS K.P.G.,
PURKERSON M.L.,
KLAHR S.
Publication year - 1991
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1991.tb01379.x
Subject(s) - saline , renal function , isotonic saline , kidney , urology , medicine , endocrinology , urinary system , excretion , diuresis
. Following 24 h of ureteral obstruction in the rat, renal blood flow and glomerular filtration rate are markedly depressed. The effect of saline loading on post‐obstructive glomerular filtration (GFR) was studied in 15 female Sprague‐Dawley rats in the awake state, 4 h following the release of 24 h of unilateral ureteral obstruction. Group I ( n = 8) received 39 μl min ‐1 of 0.9% saline only for 1 h prior to study and Group II ( n = 7) received 78 μl min ‐1 of 0.9% saline for the whole 4 h prior to study. The C in and C PAH of the post‐obstructed kidney were significantly reduced over control values in both groups. Saline loading (Group II) resulted in an improvement in C in in the post‐obstructed kidney compared with group I (3.22 ± 0.14 vs.219 ± 0.14 ml/min/kg BW, P < 0.001). This was independent of any change in C PAH . In two further groups of rats the saline loading protocol was shown to cause a rise in the excretion of urinary cGMP in the post‐obstructed kidney, but not the contralateral control kidney. In addition, administration of exogenous atriopeptin (1–24) to non‐saline loaded animals resulted in a qualitatively similar alteration in renal function to saline loading, namely a rise in C in and an increase in excretion of cGMP by the post‐obstructed kidney, and no change in C PAH . The results show that the decrease in GFR following release of unilateral ureteral obstruction may be improved by moderate saline loading possibly by enhancing the release of endogenous atrial peptide, and that the decline in GFR after release of ureteral obstruction is at least in part independent of renal plasma flow.