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Are leucocyte‐derived free radicals involved in ischaemia in human legs?
Author(s) -
CIUFFETTI G.,
MERCURI M.,
MANNARINO E.,
LOMBARDINI R.,
PASQUALINI L.,
OTT C.,
LUPATTELLI G.
Publication year - 1991
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1991.tb01367.x
Subject(s) - radical , placebo , medicine , free radical scavenger , ischemia , anesthesia , chemistry , surgery , urology , gastroenterology , pathology , biochemistry , oxidative stress , alternative medicine
. Leucocyte‐derived free radicals were monitored in 30 stage II peripheral vascular disease (PVD) patients in an open placebo‐controlled study. Linked to a transcutaneous oxygen pressure (TcPo 2 ) monitor, they performed two consecutive standard treadmill tests (5 min, 2 km h –1 12% slope) before and after 15‐days treatment with placebo or a leucocyte‐derived free radical scavenger (Piroxicam, 20 mg day –1 ), the second test being carried out at the TcPo 2 half‐recovery time. Blood samples were collected at baseline, at the maximum walking times and the TcPo 2 half recovery times. The total and differential leucocyte counts, the percentage of cells with pseudopodia or cytoplasmatic irregularities, the filterability rates (using a positive pressure Nuclepore filter system) of the main leucocyte subfractions and plasma oxidant activity were monitored. Compared with values before treatment and with the placebo‐treated group Piroxicam therapy significantly ( P < 0.001) reduced the final half‐recovery time, the percentage of cells with pseudopodia and the level of plasma oxidant activity ( P < 0.01) and kept the granulocyte filterability rate stable, showing leucocyte‐derived free radicals are involved in peripheral ischaemia.