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Regulation of myocardial isomyosin V1 in uraemic rats
Author(s) -
RAMBAUSEK M.,
KOLLMAR S.,
KLUG D.,
MEHLS O.,
RITZ E.
Publication year - 1991
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1991.tb01360.x
Subject(s) - medicine , endocrinology , muscle hypertrophy , uremia , cardiac hypertrophy , biology
. Cardiac hypertrophy of various aetiologies is consistently associated with increased expression of V3 isomyosin. Uraemia is associated with cardiac hypertrophy. In the present study, we examined regulation of isomyosin in uraemic rats, using gel electrophoresis. Cardiac hypertrophy in uraemic animals was associated with a relative increase in VI isomyosin. An increased proportion of VI isomyosin was demonstrable 3 days after subtotal nephrectomy (NX 63.0 ± 8.8%; control 43.6 ± 7.2%; P < 0.01) and persisted during uraemia of 80 days duration. Elevation of VI isomyosin, relative to pair‐fed controls, was observed in uraemic animals of various age. The proportion of VI isomyosin changed in the same direction as controls when several manouevers were used which changed the isomyosin pattern, but the difference between uraemic animals and controls persisted. We studied the effect of carbohydrate loading or deprivation, starvation or administration of energetically inadequate diets, castration or administration of androgens and sodium depletion. With each of the above interventions, a difference between subtotally nephrectomized animals and sham‐operated pair‐fed control animals was statistically significant ( P < 0.05). Elevation of VI isomyosin persisted during combined alpha and beta blockade and was still found when blood pressure was normalized by ACE inhibition using Ramipril. It is concluded that cardiac hypertrophy of uraemia differs from all other forms of cardiac hypertrophy by the occurrence of increased proportion of VI isomyosin. The proportion of VI isomyosin responds adequately to regulatory signals but is set at an abnormally high level.