Premium
Evidence for abnormal platelet glycoprotein expression in diabetes mellitus
Author(s) -
TSCHOEPE D.,
ROESEN P.,
KAUFMANN L.,
SCHAUSEIL S.,
KEHREL B.,
OSTERMANN H.,
GRIES F. A.
Publication year - 1990
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1990.tb02264.x
Subject(s) - platelet , platelet membrane glycoprotein , glycoprotein , von willebrand factor , thrombopoiesis , medicine , endocrinology , mean platelet volume , diabetes mellitus , fibrinogen , chemistry , monoclonal antibody , immunology , antibody , biology , biochemistry , microbiology and biotechnology , megakaryocyte , stem cell , haematopoiesis
In 41 diabetics (27 type I, 14 type II) and in 23 healthy controls the number of glycoprotein (GP) GPIB and GPIIB/IIIA molecules were determined on resting, peripheral platelets by means of flowcytometry after immunostaining with monoclonal antibodies which bind independently from the state of activation. The average number of both glycoproteins per platelet was significantly elevated (GPIB: 54100 times 1·27 ±1 vs. 39100 times 1·3 ±1 GPIIB/IIIA: 77500 times 1·3 ±1 vs. 62700 times 1·3 ±1 , in diabetic patients. Platelet volume was significantly correlated with the number of GPIB molecules on normal and diabetic platelets ( r (normal) = 0·52 ± 0·07; r (diabetic) = 0·46 ± 0·1). Additionally, von Willebrand factor‐related antigen (vWF: AG) was increased to 129% × 1·3 ± 1 in diabetics vs. 111% × 1·4 in controls. The increase of vWF: AG was significantly correlated with HbA 1 ( r = 0·38*) and seemed to depend on chronic hyperglycaemia. Since platelet glycoprotein receptor status is regulated by the bone‐marrow megakaryocyto‐ thrombopoiesis, our observations support the hypothesis that platelet hyperactivity in the diabetic state may be due to primarily altered production of platelets with an increased number of functional glycoproteins. This may be paralleled by increased plasma levels of cytoadhesive proteins like vWF: AG, which interact with the studied glycoproteins and thereby potentiate the risk of disturbed microhaemorrheology.