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Cyclic nucleotide phosphodiesterases and methyltransferases in purified lymphocytes, monocytes and polymorphonuclear leucocytes from healthy donors and asthmatic patients
Author(s) -
PRIGENT A. F.,
FONLUPT P.,
DUBOIS M.,
NÉMOZ G.,
TIMOUYASSE L.,
PACHECO H.,
PACHECO Y.,
BIOT N.,
PERRINFAYOLLE M.
Publication year - 1990
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1990.tb01864.x
Subject(s) - phosphodiesterase , peripheral blood mononuclear cell , cyclic nucleotide phosphodiesterase , phospholipid , monocyte , methyltransferase , lymphocyte , nucleotide , immunology , enzyme , medicine , cyclic nucleotide , endocrinology , chemistry , microbiology and biotechnology , biology , biochemistry , dna , gene , methylation , in vitro , membrane
. Cyclic nucleotide phosphodiesterase and phospholipid N‐methyl‐transferase activities were simultaneously measured in purified polymorphonuclear cell‐, mononuclear cell‐, lymphocyte‐ and mono‐cyte‐homogenates from control subjects, from patients with atopic asthma and from patients with non‐atopic asthma. Whereas cyclic AMP and cyclic GMP phosphodiesterase activities were found to be about 10‐fold lower in polymorphonuclear than in mononuclear cells, phospholipid N‐methyltransferase proved to be rather similar in each cell type from control donors. Cyclic AMP phosphodiesterase and phospholipid N‐methyltransferase were significantly decreased in polymorphonuclear cells and monocytes from asthmatic patients compared with the control group while cyclic GMP phosphodiesterase was significantly impaired only in the monocyte subpopulation.