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Lipolysis of polyenoic fatty acid esters of human chylomicrons by lipoprotein lipase
Author(s) -
EKSTRÖM B.,
NILSSON Å.,
ÅKESSON B.
Publication year - 1989
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1989.tb00228.x
Subject(s) - chylomicron , lipolysis , lipoprotein lipase , lipase , chemistry , biochemistry , fatty acid , docosahexaenoic acid , eicosapentaenoic acid , polyunsaturated fatty acid , lipoprotein , very low density lipoprotein , cholesterol , enzyme , adipose tissue
. Human chylomicrons, obtained from chylous pleural fluid of a patient with mesothelioma, were incubated with bovine milk lipoprotein lipase. No difference in the release of different C 16 –C 18 fatty acids as unesterified fatty acids, or in their appearance in diacylglycerols, was observed. The rate of lipolysis of arachidonic (20:4), and eicosapentaenoic (20:5) acid ester bonds of chylomicron triacylglycerols was slower, and a larger proportion of these fatty acids accumulated in mono‐ and diacylglycerols. The hydrolysis pattern for docosahexaenoic acid (22:6) was intermediary between that of the C 16 –C 18 fatty acids and that of 20:4 and 20:5. The data suggest that the presence of a double bond at position 5 in the eicosanoid precursors may be important for the rate of lipolysis. The relative resistance of the 20:4‐ and 20:5‐esters of chylomicrons to lipoprotein lipase may be important for the tissue distribution of these fatty acids.