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Differentiation of bone marrow cells from myelodysplastic patients in the presence of 1,25 dihydroxyvitamin D 3 or 13‐cis retinoic acid
Author(s) -
NAGLER A.,
RICKLIS I.,
GAZIT E.,
TATARSKY I.,
FABIAN I.
Publication year - 1986
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1986.tb01345.x
Subject(s) - retinoic acid , bone marrow , myeloid , incubation , tretinoin , monocyte , microbiology and biotechnology , chemistry , esterase , cellular differentiation , medicine , immunology , biology , endocrinology , biochemistry , enzyme , gene
. The separate effects of vitamin D 3 (1,25(OH) 2 D 3 ) and 13‐cis retinoic acid on the differentiation in liquid culture of marrow cells from seven patients with myelodysplastic syndrome (MDS) were studied. Following incubation with 1,25(OH) 2 D 3 ), an increasing number of myeloid cells acquired the morphological appearance of mature monocyte‐macrophages and reacted positively to fluoride‐sensitive naphthyl acetate esterase and specifically bound My 4 monoclonal antibody (McAb). Incubation of bone marrow cells with 13‐cis retinoic acid enhanced the number of cells with the morphological appearance of metamyelocytes and mature granulocytes as well as those that reacted positively with AS‐D naphthol chloroacetate esterase. The results suggest that the differentiation pattern of myeloid precursor cells from MDS patients can be modulated by 1,25(OH) 2 D 3 and 13‐cis retinoic acid.