Premium
Effects of indomethacin, prostaglandins E 2 and I 2 on the tone of human isolated femoral arteries
Author(s) -
HADHÁZY P.,
NAGY L.,
DÖMÖTÖR L.,
MAGYAR K.
Publication year - 1986
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1986.tb01337.x
Subject(s) - medicine , endocrinology , femoral artery , isometric exercise , chemistry , cyclooxygenase , basal (medicine) , blood vessel , anatomy , enzyme , biochemistry , insulin
. Strips of endothelium‐denuded femoral arteries from operated patients were set up for isometric recording. Indomethacin (IND, 3 μ mol l ‐1 ) enhanced basal tension of the arteries from 0·13 ± 0·04 to 0·86 ± 0·12 mN ( P < 0·001; n = 16) and potentiated the contractile responses of the strips to noradrenaline (NA); EC 50 for NA was 1·5 ± 0·3 μ mol l ‐1 ( n = 6) in the absence, and 0·4 ± 0·07 μ mol l ‐1 ( n = 6) in the presence of IND ( P < 0·01). PGI 2 produced dose‐related relaxation in IND‐treated vessels, its IC 50 being 15·0 ± 1·3 nmol l ‐1 . Low concentrations of PGE 2 (0·85–8·5 nmol l ‐1 ) reduced whereas its higher concentrations (28–85 nmol l ‐1 ) increased smooth muscle tone in the presence of IND. These results indicate that human femoral arteries—unlike femoral arteries of some laboratory animals—are highly sensitive to cyclooxygenase inhibition as well as to PGI 2 and PGE 2 .