Premium
Effect of immunoglobulin preparations on the aggregation of human erythrocytes
Author(s) -
MAEDA NOBUJI,
SEKIYA MISUZU,
KAMEDA KENJI,
SHIGA TAKESHI
Publication year - 1986
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1986.tb01327.x
Subject(s) - plasmin , chemistry , pepsin , antibody , facilitation , immunoglobulin g , erythrocyte membrane , red blood cell , erythrocyte aggregation , biophysics , biochemistry , immunology , membrane , biology , enzyme , neuroscience , fibrinogen
. The aggregation of human erythrocytes induced by four kinds of immunoglobulin preparations was examined by a low shear rheoscope. After removing anti‐A + and anti‐B + activities contaminated in all preparations by incubating with erythrocytes of different blood groups, the facilitating effect on the rouleau formation of erythrocytes was compared: (i) The effect of polyethyleneglycol‐treated preparation was the same in A + ‐, B + ‐, AB + ‐ and O + ‐erythrocytes. (ii) Sulfonation did not affect the velocity of rouleau formation. (iii) Some of pepsin‐treated preparations showed the strongest facilitation for A + ‐, B + ‐ and AB + ‐erythrocytes, but the facilitation was much weaker for O + ‐erythrocytes. The others showed the weak facilitation for all types of erythrocytes (especially O + ‐erythrocytes). (iv) Plasmin treatment markedly decreased the velocity of rouleau formation of AB + ‐ and O + ‐erythrocytes, but was not of A + ‐ and B + ‐erythrocytes.