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Hepatic regeneration and metabolism after partial hepatectomy in normal rats: effects of insulin therapy
Author(s) -
JOHNSTON D. G.,
JOHNSON G. A.,
ALBERTI K. G. M. M.,
MILLWARDSADLER G. H.,
MITCHELL J.,
WRIGHT R.
Publication year - 1986
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1986.tb01012.x
Subject(s) - medicine , endocrinology , insulin , hepatectomy , liver regeneration , glycolysis , glycogen , chemistry , metabolism , biology , regeneration (biology) , surgery , resection , microbiology and biotechnology
The effect of insulin therapy on liver regeneration has been studied in normal fed rats 12, 24 and 48 h after partial hepatectomy. Dry weight of regenerating liver increased between 12 and 48 h after partial hepatectomy and was unaffected by insulin therapy. [6‐ 3 H] Thymidine uptake peaked at 24‐h (24·7 ± 2·4% of total liver cells) and insulin treatment had no additional effect. At 12‐h after partial hepatectomy, hepatic [ATP] was decreased 15%, while [ADP] and [AMP] were increased 47% and 83% respectively compared with sham‐operated animals. Partial hepatectomy also caused an increase in hepatic [triglyceride], a decrease in hepatic [glycogen] and an increase in the levels of glucose and several glycolytic intermediates. The hepatic redox ratios, [lactate]:[pyruvate] and [3‐hydroxybutryate]:[acetoacetate], were elevated. Insulin therapy had only minor effects on hepatic adenine nucleotide levels, intermediary metabolite concentrations or intrahepatic redox ratios after partial hepatectomy. These findings suggest a decreased hepatic intracellular energy state in regenerating liver; insulin therapy in normal rats does not influence this metabolic change nor the regenerative response.