Premium
Production of leukotrienes and prostaglandins by human ascites cells
Author(s) -
OUWENDIJK R. J. TH.,
ZIJLSTRA F. J.,
WILSON J. H. P.,
VINCENT J. E.,
BONTA I. L.
Publication year - 1985
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1985.tb00280.x
Subject(s) - ascites , arachidonic acid , leukotriene , cirrhosis , leukotriene c4 , thromboxane , chemistry , thromboxane b2 , prostaglandin , ionophore , endocrinology , medicine , prostaglandin e , biochemistry , calcium , biology , enzyme , platelet , asthma
. Ascites was collected from six patients with liver cirrhosis and the cells isolated. These cells, mainly macrophages, were labelled with 14 C‐arachidonic acid and stimulated with the calcium ionophore A23187. The metabolites formed were separated by HPLC. The main substances formed by the ascites cells were leukotriene B 4 , 5‐hydroxy‐6,8,11,14 eicosatetraenoic acid and leukotriene C 4 . Smaller amounts of thromboxane B 2 , 12‐hydroxy‐5,8,10 heptodecatrienoic acid and 6‐keto‐prostaglandin F 1 α were isolated. Human peritoneal macrophages are therefore capable of producing leukotrienes and prostaglandins. Production of these substances might play a role in some of the complications of patients with liver cirrhosis and ascites.