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Effect of heparin and aspirin on platelet and clotting activation during leukapheresis
Author(s) -
FABRIS F.,
CASONATO A.,
RANDI M. L.,
BELLONI M.,
QUAINO V.,
ONGARO G.,
GIROLAMI A.
Publication year - 1985
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1985.tb00166.x
Subject(s) - heparin , platelet , leukapheresis , apheresis , chemistry , platelet activation , aspirin , thrombin , pharmacology , medicine , biochemistry , biology , stem cell , cd34 , genetics
. β‐thromboglobulin (βTG), serotonin (5HT) and fibrinopeptide A (FPA) were assessed in twenty‐two normal donors before, during, and at the end of leukapheresis. Seven procedures were carried out adding heparin to the circuit, and seven adding acetyl salicylic acid (ASA). The remaining eight procedures were carried out using only citrate and served as controls. Plasma βTG increased both during and after the apheresis, together with a significant decrease of the intra‐platelet content. Platelet 5HT did not show significant changes whereas FPA increased significantly. Using heparin, we obtained a complete prevention of FPA cleavage during the entire procedure, while βTG increased at the middle of the procedure. No significant effects were observed using ASA both on βTG and FPA levels. These features clearly indicate the activation of platelets and the thrombin generation in the apheresis circuit. Nevertheless, the activation of the clotting system seems to be predominant, as supported by the improved effect of heparin on βTG and FPA amounts.