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Basal cyclic AMP levels in human blood mononuclear cells
Author(s) -
SVENSON M.,
PERMIN H.,
WIIK A.
Publication year - 1984
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1984.tb01179.x
Subject(s) - theophylline , basal (medicine) , incubation , medicine , endocrinology , peripheral blood mononuclear cell , adenosine deaminase , chemistry , adenosine , amp deaminase , biology , biochemistry , in vitro , insulin
. Basal cyclic AMP levels in blood mononuclear cells from healthy human donors has been studied. A great variation in cyclic AMP content ranging from below 2 to 20 pmol 10 ‐6 cells was seen. Incubation of the cells at 37°C mostly showed a time‐dependent decrease in basal cyclic AMP, levelling off after 16 h. When treated synchronously, cells from blood from the first sample of blood sampling contained more cyclic AMP than that of later samples. Presence of adenosine deaminase, theophylline or indomethacin influenced cyclic AMP concentrations in a non‐consistent manner. Variations in cyclic AMP content may in some individuals be ascribed to early increase of cyclic AMP, in others to a progressive reduction in basal cyclic AMP during blood sampling, cell preparation and incubation.