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Effects of ethanol and acetaldehyde on cultured rabbit aortic myocytes and human platelets in vitro
Author(s) -
STAVENOW L.,
JERNTORP P.,
ÖHLIN H.
Publication year - 1984
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1984.tb01175.x
Subject(s) - acetaldehyde , ethanol , endocrinology , medicine , chemistry , in vitro , platelet , incubation , myocyte , biochemistry , biology
. The recently reported finding that a moderate alcohol consumption may lower the risk of cardiovascular disease prompted a study of the effects of ethanol and acetaldehyde on proliferation, viability and collagen secretion of rabbit aortic myocytes in culture and on the spontaneous efflux reaction of human platelets in vitro. Ethanol had no effects on any of the systems and acetaldehyde did not influence platelets significantly. Fifty μ mol l ‐1 acetaldehyde diminished the proliferation and collagen secretion of arterial myocytes by 20% ( P < 0·01) and 100 μmol l ‐1 acetaldehyde by 39% ( P < 0·001) without affecting cell mass or cell death. A metabolic degradation, and some evaporation, of acetaldehyde was taking place and 50 μmol l ‐1 acetaldehyde was halved after approximately 2 h. The more ‘physiological’ concentration of acetaldehyde (5 μmol l ‐1 ) influenced cell proliferation significantly ( P < 0·001) if the concentration was restored by 6‐h intervals and the incubation time increased from 24 to 48 h. The weak aldehydedehydrogenase inhibitor chlorpropamide did not accentuate the effects of acetaldehyde.