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The measurement of superoxide anion production by granulocytes in whole blood. A clinical test for the evaluation of phagocyte function and serum opsonic capacity
Author(s) -
VITE P. BELLA,
DRI P.,
BIANCA V. DELLA,
SERRA M. C.
Publication year - 1983
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1983.tb00114.x
Subject(s) - zymosan , superoxide , phagocytosis , phagocyte , respiratory burst , opsonin , whole blood , antibody opsonization , chemistry , immunology , biochemistry , cytochrome c , medicine , enzyme , in vitro , mitochondrion
. The paper reports a simple, sensitive and time‐saving procedure for the assay of the function of the phagocytes on microsamples of whole blood. The method consists in the evaluation of the stimulation of superoxide anion (O 2 ‐ ) production (as superoxide dismutase‐sensitive cytochrome c reduction) by leukocytes in whole blood challenged with (a) phagocytosable particles (opsonized zymosan); (b) particles that become phagocytosable by virtue of the opsonizing capacity of the plasma of blood samples (zymosan); and (c) a soluble agent such as phorbol myristate acetate. Preliminary studies indicate that this procedure can be used as a routine test because it enables information to be obtained about the respiratory responsiveness of phagocytes and about cellular and humoral defects of phagocytosis.