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Differences in β‐adrenergic receptor density and adenylate cyclase activity between normal and leukaemic leukocytes
Author(s) -
PAIETTA ELISABETH,
SCHWARZMEIER JOSEF D.
Publication year - 1983
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1983.tb00110.x
Subject(s) - adenylate kinase , cyclase , endocrinology , medicine , histamine h2 receptor , receptor , sodium fluoride , histamine , biology , hormone , chemistry , antagonist , fluoride , inorganic chemistry
. An identical class of high‐affinity binding sites for the 125 I‐labelled β‐adrenergic antagonist hydroxybenzylpindolol, was identified on intact human normal and leukaemic peripheral blood leukocytes. On normal unfractionated lymphocytes, polymorphonuclear leukocytes, and monocytes, receptor density did not differ significantly (1200–1400 receptors per cell; P > 0.3), but it was higher on B‐ than on T‐lymphocytes ( P < 005). In leukaemia, monocytic blast cells expressed highest receptor numbers, whereas very low receptor density was seen on the pathologic B‐cells from chronic lymphocytic leukaemia. Among normal leukocytes, adenylate cyclase activation by hormones (isoproterenol, prostaglandin E 1 , histamine) and sodium fluoride was strongest in plasma membranes from monocytes, but very weak in polymorphonuclear leukocytes either due to uncoupling of hormone receptors from adenylate cyclase or to low catalytic activity. In T‐cells, enzyme activity was significantly lower than in B‐cells. Loss of adenylate cyclase sensitivity to hormones and fluoride occurred in leukaemic cells from chronic and acute lymphocytic leukaemia.