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Effect of parathyroid hormone, calcitonin and growth hormone on cAMP content of growth cartilage in experimental uraemia
Author(s) -
KREUSSER W.,
WEINKAUFF R.,
MEHLS O.,
RITZ E.
Publication year - 1982
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1982.tb02242.x
Subject(s) - endocrinology , medicine , calcitonin , parathyroid hormone , cartilage , in vivo , chemistry , hormone , biology , calcium , microbiology and biotechnology , anatomy
. The response of proximal tibial growth cartilage cAMP content to different hormonal stimuli, i.e. parathyroid hormone, calcitonin and somatotropic hormone was evaluated in rats with bilateral or subtotal nephrectomy. In uraemic rats, basal cAMP content of growth cartilage was unchanged. Administration of 1–34 PTH in vivo or incubation of growth cartilage with 1–34 PTH in vitro caused a significantly smaller increment of cAMP in uraemic rats (40 IU PTH in vivo : 11·4±1·01 pmol cAMP/mg protein; controls 24·0±2·55; P <0·001). This finding implies PTH resistance. Diminished cAMP response in uraemic animals was not changed by pretreatment with 1,25(OH) 2 D 3 or parathyroidectomy. The increment of cAMP content of growth cartilage of uraemic animals was significantly ( P <0·01) greater after in vivo administration of 10 IU calcitonin (46·1±4·89 pmol/mg protein; control: 29·0±3·99) or incubation of cartilage with calcitonin in vitro . This finding implies overresponsiveness to calcitonin. Neither in acute nor in chronic uraemia, STH caused a significant change of cartilage cAMP or cGMP content, but STH stimulated 3 H‐thymidine incorporation into chondrocytes of rats with 5 days uraemia (solvent 2·98±0·51 times 10 3 cpm per cartilage; STH 5·08±0·34; P <0·05) and caused significant improvement of longitudinal growth of rats with 20 days uraemia.