Premium
The effect of lymphokines on proliferation and collagen synthesis of cultured human synovial cells
Author(s) -
PARROTT DAVID P.,
GOLDBERG RONALD L.,
KAPLAN STEPHEN R.,
FULLER GEORGE C.
Publication year - 1982
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1982.tb00688.x
Subject(s) - lymphokine , immunology , microbiology and biotechnology , chemistry , cell growth , biology , biochemistry , immune system
. Human mononuclear cell supernatants were obtained by incubating 3 times 10 6 cells per ml of Dulbecco's modified Eagles medium at 37°C for 24 h or 48 h, either in presence or absence of phytohaemagglutinin. After removal of intact cells, the supernatants were dialysed and diluted (1:1, 1:2, 1:5, 1:10) again using the above medium. The diluted supernatants, containing a final concentration of 10% (v/v) human platelet‐factor poor serum, were found to stimulate the proliferation of human synovial cells in culture and to increase both the total amount of collagen and the percentage of Type III collagen synthesized by these cells. Incubation of the mononuclear cells in presence of phytohaemagglutinin appeared to further enhance the stimulatory effects of the supernatants upon the synovial cells. Since activated mononuclear leucocytes such as lymphocytes and macrophages are present in rheumatoid synovia, this study suggests that factors released from activated mononuclear leucocytes may play an important role in the proliferation of rheumatoid synovial tissue and development of the pannus.