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Adenylate cyclase activity and cAMP content of human platelets in uraemia
Author(s) -
VLACHOYANNIS J.,
SCHOEPPE W.
Publication year - 1982
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1982.tb00683.x
Subject(s) - adenylate kinase , platelet , cyclase , medicine , endocrinology , basal (medicine) , uremia , bleeding diathesis , platelet activation , creatinine , chemistry , diabetes mellitus , stimulation , receptor
. We investigated the effects of uraemia and haemodialysis on the basal activity of adenylate cyclase and the cyclic‐AMP content of human platelets in patients with end‐stage renal insufficiency, patients receiving maintenance haemodialysis, and as controls healthy voluntary subjects. Basal adenylate cyclase activity in terminal renal disease (creatinine clearance less than 15 ml/min/1·73 m 2 ) was 824 ± SEM 57, in comparison to the healthy subjects with 453 ± SEM 28 ( P < 0·001). We also found significant elevation ( P < 0·001) of platelet cAMP levels as compared to the controls. Basal adenylate cyclase activity and platelet cAMP levels were approximately normal in the dialysed patients. These results show that uraemic toxins adversely affect the platelet AC‐cAMP system, possibly causing impaired platelet aggregation and the bleeding diathesis of uraemia.