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Lipoprotein snthesis and secretion b cultured human intestinal mucosa
Author(s) -
RACHMILEWITZ D.,
SHARON P.,
EISENBERG S.
Publication year - 1980
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1980.tb02071.x
Subject(s) - secretion , palmitic acid , triglyceride , intestinal mucosa , fatty acid , cholesterol , biochemistry , lipoprotein , chemistry , biology , medicine , endocrinology
. We tested whether cultured human jejunal mucosa incorporates fatt acid into esterified lipids and whether organ culture can serve as a model ss'em to stud lipoprotein secretion b the human gut. Jejunal biopsies obtained from seven subjects were cultured for 24 h in medium containing 2 μCi of [ 3 H]palmitic acid. More than 95% of the radioactivit incorporated b the tissue was found to be associated with esterified lipids: triglcerides, cholesterol esters and phospholipids, 7–8±1–2, 0–5±01 and 14‐0±1–5 nmol/10 mg weight respectivel. During the culture labelled triglcerides, phospholipids and cholesterol esters were secreted to the medium. Most of the newl snthesized esterified lipids in the medium were found in the d < 1 019 g/ml and d = 1019–1063 g/ml fractions. The majorit of the newl snthesized triglcerides were found in the d < 1 019 g/ml fraction. Labelled cholesterol esters were enriched in the d = 1 ‐063‐1‐21 g/ml fraction. Boiled biopsies adsorbed negligible amounts of radioactive palmitic acid and did not snthesize esterified lipids. The addition of puromcin to the culture medium and preincubation with colchicine resulted in decreased uptake of the labelled fatt acid and decreased secretion of esterified lipids to the medium. These experiments indicate that cultured human intestinal mucosa is a suitable model to stud lipid snthesis and lipoprotein secretion b the human intestine.