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Structural and functional correlations in the atrophic mucosa of self‐emptying blind loops of rat jejunum
Author(s) -
ROBINSON J. W. L.,
MENGE H.,
SCHROEDER P.,
RIECKEN E. O.,
MELLE G. VAN
Publication year - 1980
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1980.tb00051.x
Subject(s) - jejunum , microdissection , epithelium , medicine , atrophy , intestinal mucosa , endocrinology , chemistry , phenylalanine , biology , anatomy , pathology , biochemistry , amino acid , gene
. Marked atrophy of the mucosa develops in self‐emptying blind loops of rat jejunum. The application of microdissection techniques revealed a 74% reduction in the mucosal surface area of the blind loops, 3 weeks after their creation. Kinetic studies of the influx of L‐phenylalanine and β‐methyI‐D‐glucoside into rings of blind loops showed that the K m for both mechanisms was unchanged, but that there was a small reduction in the K max , for both substrates, when expressed in terms of unit weight of incubated tissue; this attained 31% for phenylalanine and 12% for β‐methyl‐glucoside. When the kinetic data were combined with the mor‐phometric results, it could be shown that the V max for both substrates, when expressed per unit area of mucosal surface, was more than twice as large in the blind loop epithelium as in normal tissue. Such findings indicate either that the individual cells of the blind loop epithelium possess more transport sites than normal. or that there is a greater proportion of absorptive cells within the blind loop mucosa.