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Metabolic activity of human polymorphonuclear leucocytes: relation to ingestion rate
Author(s) -
TORRES MARTINE,
PROST DOMINIQUE DE,
HAKIM JACQUES,
GOUGEROT MARIEANNE
Publication year - 1979
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1979.tb00925.x
Subject(s) - ingestion , hydrogen peroxide , chemistry , oxygen , superoxide , medicine , endocrinology , biochemistry , enzyme , organic chemistry
. The ingestion rate and oxygen‐dependent metabolic activities of normal human polymorphonuclear leucocytes were measured with heat‐killed Klebsiella as the particle. Since the experimental conditions were similar for each measurement, it was possible to make direct correlations between each oxygen‐dependent reaction and (1) ingestion rate and (2) the other oxygen‐dependent reactions. In the controls, oxygen‐uptake was more reliably correlated ( r = 0.960) with ingestion rates than with (in order of reliability) hydrogen peroxide produced ( r = 0.860) and iodination ( r = 0.858 and 0.813 for 100 and 20 μmol/l iodide respectively). Hydrogen peroxide production ( r = 0.988), nitroblue tetrazolium reduction ( r = 0.969) and cytochrome c reduction ( r = 0.862) were more reliably correlated to oxygen‐uptake than to ingestion rate, and iodination was better related to hydrogen peroxide production ( r = 0.90 and 0.819 for 100 and 20 μmol/l iodide respectively) than to ingestion rate. From these findings it was possible to locate primary defects in abnormal polymorphonuclear leucocytes from individual patients with pyogenic infections, idiopathic refractory anaemia or idiopathic oesteomyelofibrosis with splenomegaly, even when several deficiencies existed.