Premium
Regulation of sterol synthesis in leukaemic blast cells: a defect resembling familial hypercholesterolaemia
Author(s) -
BETTERIDGE D. J.,
KRONE W.,
FORD J. M.,
GALTON D. J.
Publication year - 1979
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1979.tb00909.x
Subject(s) - sterol , cholesterol , lipoprotein , cholesterol synthesis , precursor cell , biochemistry , medicine , biology , endocrinology , biosynthesis , familial hypercholesterolemia , chemistry , in vitro , enzyme , reductase
. Leucocytes from normal subjects and from patients with acute myeloblastic leukaemia incubated for 9 h in the absence of lipoproteins increased their rate of sterol biosynthesis from [2‐ 14 C]acetate three‐fold. Subsequent addition of complete serum, low density lipoprotein or cholesterol in a non‐lipoprotein form suppressed sterol synthesis in leucocytes from normal subjects. In contrast, complete serum or low density lipoprotein totally failed to suppress sterol synthesis in leukaemic blast cells. However, when these cells were incubated with cholesterol in a non‐lipoprotein form suppression of sterol synthesis occurred as in normal leucocytes. These results suggest that leukaemic blast cells behave like cells from patients with familial hypercholesterolaemia in showing failure of regulation of sterol synthesis by low density lipoprotein.