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A comparison between naturally occurring macroamylasaemia and macroamylasaemia induced by hydroxyethyl‐starch
Author(s) -
DÜRR H. K.,
BODE CHRISTIANE,
KRUPINSKI R.,
BODE J. CH.
Publication year - 1978
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1978.tb00835.x
Subject(s) - chemistry , sephadex , in vivo , size exclusion chromatography , amylase , hydroxyethyl starch , clearance rate , in vitro , chromatography , starch , biochemistry , enzyme , medicine , endocrinology , biology , microbiology and biotechnology
. Macroamylasaemia was produced in vitro by incubation of hydroxyethylstarch with serum, and in vivo by intravenous infusion of hydroxyethylstarch. Gel filtration on Sephadex G‐100 revealed distinct differences in molecular size distribution between such hydroxyethylstarch‐induced macroamylase and the usual form of naturally occurring macroamylase which was observed in a few patients from our hospital. Further studies demonstrated that the gel filtration elution pattern of amylase activity in serum containing hydroxyethylstarch‐induced macroamylase is significantly altered with time in vitro and in vivo , probably because of an enzymatic degradation of the hydroxyethylstarch components of the macromolecular complexes. In a healthy volunteer the serum amylase activity was elevated to a maximum of 797 u/l and the renal clearance rate of amylase was diminished to a minimum of 0.3 ml/ min after infusion of 500 ml of a 6% solution of hydroxyethylstarch, as compared to 300 u/l, and 0.95 ml/min, respectively, during the pre‐infusion period.