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Human fat cell adenylate cyclase: regional differences in adrenaline responsiveness
Author(s) -
KATHER H.,
ZÖLLIG K.,
SIMON B.,
SCHLIERF G.
Publication year - 1977
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1977.tb01657.x
Subject(s) - adenylate kinase , cyclase , adipose tissue , endocrinology , medicine , sodium fluoride , chemistry , membrane , stimulation , basal (medicine) , enzyme , biochemistry , biology , fluoride , inorganic chemistry , insulin
. The effect of adrenaline on adenylate cyclase activity in membranes prepared from human abdominal and gluteal adipose tissue was examined. Basal enzyme activity averaged 1.0 nmol cAMP/mg protein/15 min in abdominal tissue and 0.68 nmol cAMP/mg protein/15 min in ghosts from the buttock. Maximally effective concentrations of sodium fluoride (20 mmol/l) induced an eight‐fold increase in both membrane preparations. The maximal response to adrenaline (1.0 mmol/l) averaged 170% in ghosts of abdominal tissue compared with 60% in gluteal membranes. The concentrations of adrenaline required to produce half‐maximal stimulation were similar in membrane preparations of both regions (about 5 × 10 ‐5 mol/l).