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Protein synthesis in human skeletal muscle tissue: influence of insulin and amino acids
Author(s) -
LUNDHOLM KENT,
SCHERSTÉN TORE
Publication year - 1977
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1977.tb01647.x
Subject(s) - leucine , amino acid , phenylalanine , insulin , medicine , endocrinology , stimulation , protein biosynthesis , skeletal muscle , protein metabolism , metabolism , biology , chemistry , biochemistry
. The effects of a mild stimulation of endogenous insulin secretion on the rate of incorporation in vitro of amino acids into human skeletal muscle protein were compared with the effects on this rate of adding insulin and amino acids in vitro . An intravenous infusion of 0.83 mmol glucose per kg body weight increased both plasma glucose and insulin levels significantly. The rate of incorporation of leucine into protein in isolated muscle fibres was significantly higher if the subjects had received the glucose infusion. Both insulin and a high concentration of amino acids in the incubation medium stimulated this rate. Amino acids stimulated the rate more than insulin and insulin did not further stimulate the amino acid‐stimulated rate. On the other hand, the stimulation of the rate of incorporation by amino acids was the same whether the subjects had received saline and were thus in a basal state or whether they had been infused with glucose (which itself stimulated the rate). The rate of incorporation of leucine was correlated with the tissue concentration of RNA but the correlation was weaker when the rate was stimulated by amino acids than under other experimental conditions. Leucine stimulated the rate of incorporation of phenylalanine but phenylalanine did not stimulate the rate of incorporation of leucine. It is concluded that the rate of incorporation rate of amino acids into protein in isolated human skeletal muscle fibres is subjected to hormonal and amino acid control.