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Modifications in the Release of Rat Growth Hormone in vitro and the Morphology of Rat Anterior Pituitaries Incubated in Various Ionophores
Author(s) -
MiraMoser F.,
Schofield J.G.,
Orci L.
Publication year - 1976
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1976.tb00499.x
Subject(s) - in vitro , morphology (biology) , chemistry , medicine , endocrinology , microbiology and biotechnology , biology , biochemistry , zoology
. The ionophore A23187 increased the release of rat growth hormone in the presence of a phosphodiesterase inhibitor, 3‐isobutyl‐l‐methylxanthine; a second ionophore X537A inhibited growth hormone release induced by the methylxanthine. A23187 did not alter rat growth hormone release in the absence of 3‐isobutyl‐l‐methylxanthine, but X537A enhanced hormone release in the absence of calcium or in the presence or somatostatin. These findings provide further evidence that both calcium and cyclic AMP are important in the regulation of growth hormone release. Tissue incubated in X537A contained electron‐lucent vesicles apparently derived from the Golgi apparatus, swollen granules and mitochondria with dense matrices. Tissue incubated in the presence of valinomycin or A23187 did not show altered morphology of either secretory granules or the Golgi complex. Possible mechanims of these changes are discussed.