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Synthesis of Immunoglobulins and 15 Other Proteins by Diseased Human Kidneys
Author(s) -
Poortmans J.,
Bienenstock J.
Publication year - 1975
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1975.tb00466.x
Subject(s) - antiserum , antibody , lactoferrin , immunoglobulin d , secretory component , incubation , transplantation , blood proteins , biology , immunoglobulin e , microbiology and biotechnology , biochemistry , immunofluorescence , chemistry , immunology , medicine , b cell
Summary. In an effort to determine the synthetic capacity of human kidneys for serum proteins, pieces of both kidneys removed at operation prior to transplantation were placed in organ culture. Incubation with 14 C labelled amino acids and subsequent radioimmunoelectrophoresis with antisera to all five classes of immunoglobulins showed synthesis of IgG by all 5 pairs of kidneys of IgA and IgE by 4, and of IgM by 2. None synthesized IgD. Radioimmunoelectrophoresis with specific antisera to 15 non‐immunoglobulin proteins revealed synthesis of lactoferrin and uromucoid while several other serum proteins, such as al antitrypsin and α2 macroglobulin, known for their binding properties, showed apparent synthesis. No evidence for secretory component synthesis was seen despite positive immunofluorescent localization in the tubular cells. Further, Zn α2 and β 2 GP I also showed positive immunofluorescence but negative synthesis. It seems possible that these latter proteins may not be synthesized by the tubular cells but rather may be actively excreted or reabsorbed by them.