Premium
Dynamics of Insulin Release and Microtubular‐Microfilamentous System
Author(s) -
Obberghen E.,
Devis G.,
Somers G.,
Ravazzola M.,
MalaisseLagae F.,
Malaisse W. J.
Publication year - 1974
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1974.tb02350.x
Subject(s) - colchicine , cytochalasin b , insulin , medicine , endocrinology , intracellular , microtubule , gliclazide , chemistry , biophysics , biology , microbiology and biotechnology , cell , biochemistry
. Colchicine failed to inhibit and actually enhanced insulin release evoked by gliclazide at a low glucose concentration (0.3 mg/ml). The facilitating action of colchicine was invariably present, whatever the concentration of (2.10 −5 to 10 −3 M) and length of exposure to (25 to 120 min.) the mitotic spindle‐inhibitor. Colchicine, and similarly cytochalasin B, also minimized the reduction in insulin output otherwise observed during the second of two successive stimulations with sulphonylurea. These data suggest that the short‐lived secretory response evoked by sulphonylurea corresponds to a modality of insulin release which does not require the oriented intracellular transport of secretory granules.