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The Relation between Plasma Cholesterol and Cholesterol Synthesis in Rats with Experimental Biliary Obstruction
Author(s) -
Calandra S.
Publication year - 1973
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1973.tb02204.x
Subject(s) - medicine , cholesterol , endocrinology , cycloheximide , ligation , small intestine , cholesterol synthesis , bile duct , reverse cholesterol transport , biology , chemistry , biochemistry , enzyme , protein biosynthesis , lipoprotein , reductase
Summary. The relationship between serum cholesterol and the rate of cholesterol synthesis in liver and intestine was studied in rats with experimental ligation of the bile duct. In the acute stages of biliary obstruction the level of plasma cholesterol increased several fold above the control level and was accompanied by a similar rise in the rate of hepatic and intestinal cholesterogenesis. Feeding a high cholesterol diet before the ligation of the bile duct resulted in a marked reduction in the absolute rate of cholesterol synthesis in both liver and intestine of obstructed rats, but it had no effect on the plasma cholesterol level. The administration of cycloheximide completely prevented the increase in hepatic and intestinal cholesterogenesis in obstructed rats but did not cause a fall in the level of total plasma cholesterol. These results would suggest that the accumulation of cholesterol in the plasma of rats with acute biliary stasis is not dependent upon the increased rate of cholesterol synthesis in liver and intestine.