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Cytochemical Population Analyses of Glycogen in Neutrophil Leukocytes of Chronic Myelocytic Leukaemia during Busulfan Treatment
Author(s) -
Gahrton Gösta,
Zetterberg Anders
Publication year - 1972
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1972.tb00670.x
Subject(s) - glycogen , busulfan , population , cell , biology , endocrinology , medicine , biochemistry , chemotherapy , environmental health , cyclophosphamide
A comparative investigation of glycogen content and glycogen synthesis in neutrophil leukocytes from patients with untreated chronic myelocytic leukaemia (CML) and CML in remission was undertaken using microspectrophotometry of the periodic acid‐Schiff (PAS) reaction and autoradiography after glucose‐H 3 incorporation into glycogen of single cells. The glycogen content was low and the rate of glycogen synthesis high in neutrophils from untreated CML, indicating an increased glycogen turnover rate in these cells compared to normal neutrophils. Both glycogen content and rate of glycogen synthesis reached normal levels in CML neutrophils in busulfan induced remission. This normalization of glycogen metabolism was not observed until WBC approached normal values after several weeks of treatment. This indicates that the effect of busulfan on normalization acted at the level of the myeloid precursor cell. The mechanism of normalization may be due to busulfan partially or completely eradicating a cytochemically abnormal leukaemic cell population and thereby “unmasking” a cytochemically normal cell population.