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Measurement of Plasma 25‐Hydroxycholecalciferol in Man
Author(s) -
Bayard Francis,
Bee Philippe,
Louvet J. Pierre
Publication year - 1972
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1972.tb00644.x
Subject(s) - chemistry , incubation , vitamin d and neurology , chromatography , calcifediol , human plasma , vitamin , plasma concentration , plasma , medicine , endocrinology , vitamin d deficiency , biochemistry , physics , quantum mechanics
. A radio‐ligand assay for the measurement of 25‐hydroxycholecalciferol (25‐OH D 3 ) in human plasma has been devised. Three ml of plasma, with 25‐hydroxychole‐calciferol‐26, 27‐H 3 ( 3 H‐25‐OH D 3 ) added for recovery, was extracted and purified by thin‐layer chromatography. The plasma from an osteomalacic man, diluted 1 to 800, was used as a source of binding proteins. After overnight incubation at 4° C bound and free fractions were separated using Florisil. The method has no blank and high sensitivity (2 ng). Plasma concentration of 25‐OH D 3 in normal subjects was 1.50±0.42 (mean ±SD, μg/100ml); in vitamin D deficient patients the values ranged from 0.2 to 0.7 (μg/100 ml.