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The Effect of Adult Diabetes on Glucose Utilization and Esterification of Palmitate by Human Adipose Tissue
Author(s) -
Galton D. J.,
Wilson J. P. D.,
Kissebah A. H.
Publication year - 1971
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1111/j.1365-2362.1971.tb00548.x
Subject(s) - adipose tissue , medicine , endocrinology , chemistry , diabetes mellitus , carbohydrate
. 1. The conversion of glucose and palmitate to neutral lipid by adipose tissue has been studied in a group of 30 adult diabetics and compared to a group of 30 non‐diabetic controls. — 2. Adipose tissue from both sources showed similar saturation characteristics for glucose; uptake of palmitate was linear up to 3 mM. — 3. Adipose tissue of diabetics incubated with glucose (14 mM) converted 270 ± 60 (90) mμmoles/mg protein/2 hours to neutral lipid compared to non‐diabetics who converted 380 ±70 (10) mμmoles/mg protein/2 hours. Similar results were obtained with a medium glucose concentration of 8 mM. No differences were found in glucose utilization between a group of 7 lean diabetics and 6 obese diabetics. — 4. Conversion of palmitate to neutral lipid by adipose tissue was inhibited by cyanide (1.5 mM), fluoride (50 mM) and was found to be temperature dependent. — 5. Adult diabetics converted 0.36 ± 0.06 (17) mμmoles palmitate/mg protein/2 hours into neutral lipid, compared to a group of non‐diabetics who converted 1.09 ± 0.35 (13) mμmoles/mg protein/2 hours.— 6. The significance of these observations is discussed in relation to the impaired glucose tolerance test of diabetes.