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Determination of cycle cut off in real‐time PCR for the detection of regulated plant pathogens
Author(s) -
Chandelier A.,
Planchon V.,
Oger R.
Publication year - 2010
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2009.02354.x
Subject(s) - plant quarantine , detection limit , quarantine , real time polymerase chain reaction , computer science , statistics , biology , mathematics , gene , ecology , genetics
Given the high importance of an accurate detection for quarantine plant pathogens, and the risk of false positive or false negative results, a detection method must be correctly validated. A statistical procedure has been developed to determine the cycle cut off and the corresponding limit of detection in real‐time PCR, considering a risk of false positive results of 1% and a probability of detection of 95%. The procedure presented in this paper is easy to develop and can be applied to any plant pathogen to be detected by real‐time PCR.