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Molecular characterization of a morphologically unusual potato cyst nematode*
Author(s) -
Reid A.,
Pickup J.
Publication year - 2005
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2005.00810.x
Subject(s) - globodera rostochiensis , potato cyst nematode , biology , globodera pallida , restriction fragment length polymorphism , nematode , cyst , restriction enzyme , internal transcribed spacer , ribosomal dna , spacer dna , ribosomal rna , botany , polymerase chain reaction , dna , genetics , solanaceae , ecology , phylogenetics , gene , pathology , medicine
During routine screening of soil samples for potato cyst nematodes, a morphologically variant cyst was detected. The internal transcribed spacer regions of the ribosomal DNA repeat unit from individual juveniles hatched from the cyst were amplified by PCR. The resulting products were digested with a range of restriction enzymes and their RFLP profiles compared with standard samples of Globodera rostochiensis and Globodera pallida . Most of the RFLP profiles yielded patterns similar to those of G. pallida although some restriction enzymes gave hybrid patterns showing elements of both G. rostochiensis and G. pallida . Clones of individual ITS regions from a single nematode from the variant cyst displayed either a G. pallida or G. rostochiensis pattern.