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Development of RT‐PCR assays using fluorogenic‐3′ minor groove binder DNA probes for detection of fruit tree viruses *
Author(s) -
Roussel S.,
Kummert J.,
Salmon M.,
Dutrecq O.,
Jijakli M. H.
Publication year - 2005
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2005.00803.x
Subject(s) - prunus , biology , horticulture , phytosanitary certification , virology , botany
In phytosanitary certification, there is currently a need for the development of reliable, sensitive and rapid tests for the routine detection of Ilarviruses and latent viruses from fruit trees during the dormant season. We have developed real‐time RT‐PCR assays that allow the reliable detection of Prunus necrotic ringspot ilarvirus , Apple chlorotic leafspot trichovirus and Apple stem pitting virus in bark tissues of dormant wood. These assays are well adapted for the routine detection of these three viruses because they eliminate one risk of contamination by performing the whole test in a single closed tube.