Premium
Detection of Meloidogyne incognita and Pochonia chlamydosporia by fluorogenic molecular probes *
Author(s) -
Ciancio A.,
Loffredo A.,
Paradies F.,
Turturo C.,
Sialer M. Finetti
Publication year - 2005
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2005.00794.x
Subject(s) - meloidogyne incognita , terra incognita , biology , nematode , fungus , botany , ribosomal dna , gene , genetics , ecology , phylogenetics
Fluorescent molecular probes were applied for detection of the plant parasitic nematode Meloidogyne incognita and the nematode‐egg parasitic fungus Pochonia chlamydosporia var. chlamydosporia . A region in the M. incognita rDNA including ITS2 was selected for amplification and recognition with a real‐time PCR assay, based on a combination of three specific motifs, each recognized by a specific fluorescent probe. Similarly, a Scorpion probe was designed for the RT‐PCR quantification of P. c . chlamydosporia . For this purpose, the ITS‐2 rDNA gene of the fungus was sequenced from a number of Italian isolates. A conserved region unique for P. c . chlamydosporia found in the ITS‐2 rDNA gene was used. The probes allowed recognition of single juveniles of M. incognita and of the mycelium‐ or soil‐extracted fungal DNA. The potentialities of the detection procedures are discussed.