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Diagnostics of cyst nematodes and tephritid fruit flies using mitochondrial and ribosomal DNA *
Author(s) -
Fleming C. C.,
Rao J.,
Moreland B.,
Craig D.,
Turner S. J.
Publication year - 2000
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2000.tb00952.x
Subject(s) - biology , ribosomal dna , restriction fragment length polymorphism , internal transcribed spacer , mitochondrial dna , ribosomal rna , polymerase chain reaction , pest analysis , dna barcoding , phytosanitary certification , tephritidae , botany , zoology , genetics , gene , horticulture , phylogenetics
The accurate identification of invertebrates intercepted by European phytosanitary authorities is an important step in monitoring and controlling the movement of potential pests of EU agriculture, forestry and horticulture. Nematodes and tephritid fruit flies pose particular problems, with many intercepted specimens not identified to species level. Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) analysis of mitochondrial and ribosomal DNA genes have been used to assess interspecific variability in the Tephritidae and in cyst nematodes, thus developing more effective diagnostic procedures for these invertebrate pests. PCR‐RFLP targeting the ribosomal DNA and mitochondrial DNA can be used to identify 10 tephritid species commonly detected in imported materials. Similarly, rDNA variation can be used to discriminate routinely between cyst nematodes of the genera Heterodera, Punctodera and Globodera. The integration of traditional morphology‐based diagnostics with a molecular approach is regarded as the most effective way of enhancing phytosanitary diagnostics in the future.