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Reliable identification of the quarantine root‐knot nematodes Meloidogyne chitwoodi and M. fallax by PCR‐based techniques *
Author(s) -
Zijlstra C.
Publication year - 2000
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2000.tb00950.x
Subject(s) - quarantine , biology , knot (papermaking) , horticulture , botany , ecology , engineering , chemical engineering
The root‐knot nematodes Meloidogyne chitwoodi and M. fallax have been declared quarantine pests in Europe and there is a growing need for practical, reliable techniques to identify these nematodes and distinguish them from M. hapla. Such methods are needed for plant resistance management and research, for design of crop rotation systems and for inspection of harvested crops or soil samples. At the moment, several PCR‐based techniques have been developed, based on rDNA and SCAR primers. Some of these methods can be applied for extremely sensitive detection of a specific species, enabling the detection of 0.001 juvenile or less. These can also detect specific species present in mixtures in proportions of less than 1%. Others enable a precise estimation of the species composition in mixtures and can detect species present in mixtures in proportions as low as 5%. Depending on the question to be asked and the source of the sample to be studied, optimal diagnostic assays can be created by combining relevant methods.